Cell Lines and Cell Cultures
Cell Biolabs Inc A549/GFP Cell Line
Cell Biolabs' A549/GFP Cell Line stably expresses GFP which was introduced into parental A549 lung cancer cells using lentivirus. This cell line otherwise exhibits the same characteristics of the parental A549 cell line.
Cell Biolabs Inc NIH3T3/GFP Cell Line
Cell Biolabs' NIH3T3/GFP Cell Line stably expresses GFP and blasticidin-resistant genes. Both GFP and blasticidin-resistant genes were introduced into parental NIH3T3 cells using lentivirus. This cell line otherwise exhibits the same characteristics of the parental NIH3T3 cell line.
Promocell GmbH PromoCell HWP Pellet from > 1 million Normal Human White Preadipocytes, visceral
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Promocell GmbH PromoCell HPASMC-c 500,000 Human Pulmonary Artery Smooth Muscle Cells, cryopreserved
Primary Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are isolated from human pulmonary arteries and stain positive for smooth muscle α-actin. They are suited for studying the function of pulmonary arteries under normal and disease conditions (e.g. vasoconstriction induced by alveolar hypoxia). They are also useful for physiological studies of vascular smooth muscle cells.
Promocell GmbH PromoCell HSAEpC-c 500,000 Human Small Airway Epithelial Cells, cryopreserved
Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area. They stain positive for cytokeratin.The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.Our HSAEpC are now also available from HLA-typed and COPD/Asthma donors.Available formats:* Cryopreserved: Cryogenic vial containing 500.000 viable cells.* Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
Promocell GmbH PromoCell HAoAF-c 500,000 Human Aortic Adventitial Fibroblasts, cryopreserved
Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.
Promocell GmbH PromoCell HDLEC-c 500,000 Human Dermal Lymphatic Endothelial Cells, adult, cryopreserved
Primary Human Dermal Lymphatic Endothelial Cells (HDLEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining:> 95% of the cells are CD31 positive and podoplanin positive.Lymphatic vessels transport excess fluids from tissues to the circulatory system and are a major component of the immune system. The transported fluid is nearly cell free. The vessels are highly permeable, because they lack a continuous basal membrane. Lymphatic endothelial cells are involved in pathological alterations of the lymphatic system. During tumor lymphangiogenesis, the lymphatic endothelial cells build new vessels that infiltrate tumors, attract tumor cells, and induce tumor cell metastasis.
THP-1-CD16A (ATCC CRL-3575) human monocyte-like suspension cell line, puromycin-selected
THP-1-CD16A is a human monocyte-like suspension cell line transduced to express human Fc gamma receptor IIIA (CD16A) and the Fc receptor gamma chain. It is used to study Fc receptor-mediated phagocytosis and immune effector functions and is maintained under puromycin selection.
- Human monocyte-like suspension cell line engineered to express CD16A.
- Maintained in RPMI-1640 supplemented with 10% fetal bovine serum.
- Requires puromycin selection at 5 μg/mL to retain receptor expression.
- Culture at 37 °C in a humidified incubator with 5% CO2.
- Cryopreserve in complete medium with 5% DMSO; recommended ≥ 5.0 x 10^6 cells per vial.
- Suitable for phagocytosis and Fc receptor functional assays.
ATCC Mycobacterium avium; Strain K-10; genomic DNA
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR).
STEMCELL Technologies Human Cord Blood CD19+ B Cells, Frozen, Size: 2.5 x 10^6 cells
Primary human CD19+ B cells were isolated from umbilical cord blood (CB) mononuclear cells (MNCs) using positive immunomagnetic separation techniques. CB was collected using citrate-phosphate-dextrose (CPD) as an anticoagulant. CD19 is primarily expressed on B cells.Cells were collected using consent forms and protocols approved by either an Institutional Review Board, the Food and Drug Administration, the U.S. Department of Health and Human Services, and/or an equivalent regulatory authority.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.
Revvity Health Sciences Inc Tag-lite κ Opioid Receptor (OP2) Frozen & Labeled Cells, 200 Assay Points
Tag-lite κ Opioid Receptor (OP2) Frozen & Labeled Cells, 200 Assay Points
Revvity Health Sciences Inc HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
HTRF Tag-Lite Angiotensin Receptors Red ago. Ligand, 5,000 Assay Points
STEMCELL Technologies G-CSF Mobilized Human Peripheral Blood Mononuclear Cells, Frozen, Size: 2.5 x 10^7 cells
Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications. Donors are mobilized with G-CSF, plerixafor, or G-CSF and plerixafor prior to collection. Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in CryoStorCS10 medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. ACDA is added as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.